Table 8 Primer sequences used for qRT‒PCR.

From: Effects of organic zinc on production performance, meat quality, apparent nutrient digestibility and gut microbiota of broilers fed low-protein diets

Gene

Primer nucleotide sequence (5′ → 3′)

Product length (bp)

Reference

PepT1

F: TTCCCATGGAGTCAACAGGC

R: GGCTGCTGCATTCTTGATGG

146

Yu et al.66

SGLT1

F: TGTGGGCATAGCAGGAACAG

R: TACTCCGGCATTGTCACCAC

141

Yu et al.66

MCT4

F: GCTGGTCTCAAGTGGGTTAG

R: CCACCGTAATCGACAGACATAG

107

Zhao et al.67

MCT1

F: GTGACCATTGTGGAGTGCTG

R: CCACAGGCCCAGTATGTGTA

101

Qu et al.68

β-Actin

F: ACCGGACTGTTACCAACACC

R: CCTGAGTCAAGCGCCAAAAG

116

Yu et al.66

  1. F, forward; R, reverse; PepT1, peptide transporter 1; SGLT1, sodium glucose cotransporter 1; MCT4, monocarboxylate transporter 4; MCT1, monocarboxylate transporter 1.
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