Figure 3
From: Evaluation of nimotuzumab Fab2 as an optical imaging agent in EGFR positive cancers
Binding of nimotuzumab Fab2 to cells and serum stability. Fab2 and IRDye800CW-labeled Fab2 were titrated with A-431 cells and analyzed by flow cytometry to obtain a binding constant to EGFR. (a) shows a representative histogram of the mean fluorescence intensity (MFI) of the unlabeled Fab2 at each concentration tested. (b) shows the titration curves produced from plotting the concentration vs normalized MFI to obtain percent bound. (c) serum stability of IRDye800CW-labeled Fab2 was tested at 37 °C, run on SDS-PAGE and analyzed for IRDye800CW fluorescence.
