Table 2 Antimicrobial activities of the extracts containing the different sterols against three test organisms.

Code	Sample\test organism	Conc. (mg/disc)	Inhibition zones (diameter, mm)
			B. subtilis		Methicillin-susceptible S. aureus 25923		E. coli 25922
			A	B	A	B	A	B
1	Long-horned grasshoppers (R. differens)	0.2	6.33 ± 0.58^ab	6.33 ± 0.58^b	7.67 ± 0.58^ad	0 ± 0.00^a	7.0 ± 1.00^bc	0 ± 0.00^a
2	African fruit beetle (P. sinuata)	0.2	7.33 ± 0.58^ac	6.67 ± 0.58^b	6.33 ± 0.58^ab	7.0 ± 0.00^b	6.33 ± 0.58^bc	7.67 ± 0.58^b
3	Cricket (S. icipe)	0.2	8.33 ± 0.58^c	0 ± 0.00^a	6.67 ± 0.58^ac	0 ± 0.00^a	0 ± 0.00^a	7.0 ± 1.00^b
4	Caterpillar (G. krucki)	0.2	6.33 ± 0.58^ab	6.33 ± 0.58^b	6.67 ± 0.58^ac	7.0 ± 0.00^b	7.67 ± 0.58^bc	6.33 ± 0.58^b
5	Termite (Macrotermes sp.)	0.2	7.67 ± 0.58^bc	7.33 ± 0.58^bc	7.0 ± 1.00^ad	6.67 ± 0.58^b	7.67 ± 0.58^bc	7.33 ± 0.58^b
6	Black Soldier Fly (H. illucens)	0.2	8.0 ± 0.00^c	7.67 ± 0.58^bc	8.67 ± 0.58^b	0 ± 0.00^a	8.0 ± 1.00^c	7.33 ± 0.58^b
7	Locust (S. gregaria)	0.2	7.0 ± 0.00^ac	8.67 ± 0.58^c	8.0 ± 1.00^bcd	7.0 ± 0.00^b	8.0 ± 1.00^c	6.67 ± 0.58^b
8	Cricket (G. bimaculatus)	0.2	7.33 ± 0.58^ac	6.67 ± 0.58^b	8.33 ± 0.58^cd	6.33 ± 0.58^b	6.67 ± 0.58^bc	6.67 ± 0.58^b
9	Silkworm (B. mori)	0.2	7.67 ± 0.58^bc	6.33 ± 0.58^b	7.33 ± 0.58^ad	6.33 ± 0.58^b	6.67 ± 0.58^bc	0 ± 0.00^a
Pos:	Oxy*	0.02	21.0 ± 1.00^d	19.67 ± 1.16^d	8.33 ± 0.58^cd	7.0 ± 0.00^b	20.33 ± 0.58^d	25.0 ± 1.00^c
Neg:	A. DCM	–	6.0 ± 0.00^a	–	6 ± 0.00a	–	6 ± 0.00b	–
	B. 20% ACN	–	–	n.i	–	n.i	–	n.i
F value			152.8	217.1	5.729	386.1	149.5	389.0
p value			< 2 × 10^–16	< 2 × 10^–16	3.18 × 10^–4	< 2 × 10^–16	< 2 × 10^–16	< 2 × 10^–16

Mean values of triplicate inhibition zones and their standard deviations. Values with the same superscript in the same column are not significantly different at p < 0.05 and d.f (10, 22). *Positive controls used (Oxy = Oxytetracycline), Negative controls (DCM dichloromethane, 20% ACN acetonitrile supplemented by 0.1% DCM), n.i no inhibition samples using 20 µL of the given concentration (10 mg/mL) solution. The inhibition values for positive controls were obtained with 20 µL of 1 mg/mL solution. All the values are means of triplicate experiments and their standard deviations.

Search