Figure 17

The effect of the cell overlapping on the detection sensitivity. With no overlap in the cell signals (black line), the detection sensitivity starts to decrease exactly at the optimal slice thickness point. With the overlap, the sensitivity linearly decreased started from the beginning (X = 1 µm) until reaching the optimal slice thickness (X = 44 µm) where the sensitivity started to decrease hyperbolically. With higher degree of cell overlapping (as determined by Density), the sensitivity decreased at a higher rate. Although the optimal points are well-defined in all situations (at X = 44 µm), the perfect sensitivity could not be maintained in all situations (Sens < 100%).