Table 2 List of reagents used in the DEST assay.
Reagent | Company | Catalog # | Stock conc | Final conc |
|---|---|---|---|---|
Bovine serum albumin | Fisher Scientific | BP1605-100 | – | 2% w/v |
UltraBlock | Bio-Rad | BUF033C | – | 10–100% |
ELISA general assay diluent | Bio-Rad | BUF037A | – | use neat |
HAMA Blocker | Abcam | ab193969 | – | use neat |
PBS | Thermo Scientific | 70011069 | 10X | 1X |
Tween-20 | Sigma-Aldrich | P9416-100 mL | 100% | 0.1% |
Pooled normal human plasma (K2 EDTA) | Innovative Research Inc | IPLA-N | – | 1-10 μl |
Streptavidin-HRP | Thermo Scientific | 21130 | 1.1 mg/mL | 137.5 ng/mL |
Biotinyl tyramide | Sigma-Aldrich | SML2135-50 mg | 2 mg/mL (DMSO) | 5 μg/mL |
Borate buffer | Thermo Scientific | 28341 | 20X | 2X |
Hydrogren peroxide solution | Sigma-Aldrich | H1009-100 mL | 30% | 0.003% |
Brilliant violet 421 streptavidin | BioLegend | 405225 | 0.5 mg/mL | 0.5 μg/mL |
