Figure 10
Fluorescence spectra of HeLa cells under 450 nm excitation. (A) Fluorescence spectra of water, PBS, HeLa cells diluted in PBS and Atto488 (1 μM in PBS). (B) Enlarged fluorescence spectra of water, PBS, HeLa cells diluted in PBS. (C) Normalized to the maximum intensity and adjusted for the Raman scattering fluorescence spectrum of HeLa cells diluted in PBS. (D) Normalized to the maximum intensity fluorescence spectrum of Atto488 (1 μM in PBS). Graphs were created with Excel 365.
