Figure 2

Autofluorescence of HeLa cells. (A) Autofluorescence of HeLa cells at 100 ms and 2000 ms exposure time. Integrated density (IntDen) was measured for the background (1) and cell region (2) using ImageJ software (NIH, US). Signal-to-background ratio (S/Bk) was calculated for image selections. (B) Enlarged picture represents tubular (white arrows) and round (red arrows) intracellular structures. 2000 ms exposure time. (C) Vesicle tracking within the time range, 0–20 s. Blue color represents vesicle movement through the time-lapse image (Movie S3).