Figure 3
Colocalization of autofluorescence signal in HeLa cells. (A) Cells were labeled with fluorescent probe LysoTracker. Labeled cells were imaged by confocal microscopy for LysoTracke (100 ms exposure time) and autofluorescence signal (2000 ms exposure time). The digital images were processed using ImageJ software (NIH). Yellow arrows show colocalization of autofluorescent signal with lysosomal probe. White arrows indicate no colocalization of autofluorescent signal with lysosomal probe. (B) Cells were labeled with fluorescent probe MitoTracker. Labeled cells were imaged by confocal microscopy MitoTracker (100 ms exposure time) and autofluorescence signal (2000 ms exposure time). The digital images were processed using ImageJ software (NIH). Yellow arrows show colocalization of autofluorescent signal with mitochondrial probe. White arrows indicate no colocalization of autofluorescent signal with mitochondrial probe.
