Figure 6

Signal-to noise analysis of the imaging system. (A) 505 nm laser spot irradiation was performed using taper ~ 15 µm in diameter. Dotted circle highlights the region of signal integration where intensity within time-frame of 25 s was assessed by ImageJ software (NIH). (B) The integrated density changes during the measurement as assessed by ImageJ software (NIH). The zoom-in data shows camera noise. (C) Representation of ranges of autofluorescence decay of cells as assessed by ImageJ software (NIH) and CellSens software (Olympus, Japan). Graphs were created with SigmaPlot 13.0.