Figure 2

Cellular morphology and proliferation alteration under light microscope before and after ELF-EMF exposure. (A) After five days, the shape of cancerous cells which were exposed to the ELF-EMF altered and the rate of rounded cells (which may be interpreted as cells undergo apoptosis) was much more in them (fifth day, exposed U87 and U251); these shapes were rare in the fibroblasts. Comparing the two cancerous cells, the rate of rounded cells was much more in U87, furthermore, it seemed that cancerous cells weren’t able to proliferate as much as their sham exposure group. (B) Trypan blue results for the fifth day revealed that after exposure the total number of cells in the exposed groups is less compared with their corresponding sham exposure (the same number of cells were seeded for each exposed and sham exposure group (the first day: just before exposure)). This decrease was statistically significant in fibroblast, U251, and U87 cells (*: p-value < 0.05; **: p-value < 0.01); however, in the U251-P53 group this increase was not statistically significant (Mann–Whitney U test performed as statistical analysis to compare each exposed group with its sham exposure).