Table 1 Primes sequence used for real-time PCR.
Gene name | Accession number | Primers Sequence (5' to 3') | Amplicon length(bp) |
|---|---|---|---|
P53 | NM-000546 | F: TGTGACTTGCACGTACTCCC R: ACCATCGCTATCTGAGCAGC | 199 |
P21 | NM-000389 | F: GACCATGTGGACCTGTCACT R: CGTTTGGAGTGGTAGAAATCTGTC | 140 |
MDM2 | NM-001145337 | F: TCTGTGAGTGAGAACAGGTGTCR: TGGCGTTTTCTTTGTCGTTCA | 191 |
MCM6 | NM-005915 | F: CAGCATTAAAGAGGAGCGAGC R: GCTCTCACTTCCCTCTGTGG | 191 |
CCNB1 | NM-001354844 | F: GCACTTCCTTCGGAGAGCAT R: TGTTCTTGACAGTCCATTCACCA | 188 |
