Figure 2 | Scientific Reports

Figure 2

From: The kynurenic acid analog SZR104 induces cytomorphological changes associated with the anti-inflammatory phenotype in cultured microglia

Figure 2

Immunocytochemical localization of microglial cells in secondary cultures. Secondary, microglia-enriched cultures were prepared and maintained as described in the Materials and Methods. A total of 974 cells were counted under a fluorescence microscope using a 20× or 40× objective. Representative immunocytochemical pictures from unstimulated/control (A–D), LPS- (E–H), KYNA- (I–L), LPS + KYNA- (M–P), SZR104- (Q–T) and LPS + SZR104-treated cultures (U–X). Note the varying levels of morphological heterogeneity of the microglial cells in control (unchallenged) and treated cultures. SZR104-treated cultures included mostly hypertrophied microglia (Q–T). Scale bar: 75 μm.

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