Figure 4
TOP2A regulates the proliferative capacity of non-small cell lung cancer. (A,B) Stable overexpression and knockdown systems of TOP2A identified by WB and qPCR. Some blot strips were cut prior to hybridization with the antibody, and the original blot strips are listed in the supplemental material (Supplementary Original western blot images). (C,D) Proliferation capacity of A549 and H1299 cells after overexpression or knockdown of TOP2A was detected by CCK-8 assay. (E–H) Colony formation analysis of non-small cell lung cancer cell growth. Data were determined by three replicate experiments (mean ± standard deviation).
