Figure 6
TOP2A expression was significantly correlated with PD-L1 expression and VM formation in NSCLC tissues. (A) The presence of VM was detected by CD31/PAS double staining. CD31+PAS− channels are endothelium-dependent vessels (red arrows). (B) CD31−PAS+ channels are considered VM (blue arrows), in which erythrocytes are visible (black arrows). (C) Typical NSCLC adjacent to normal and tumor tissue specimens with representative TOP2A staining (400 ×). (D) Immunohistochemical methods to detect VM expression in TOP2A-positive and TOP2A-negative groups. (E) Kaplan–Meier survival analysis showed poor overall survival in patients with high VM expression. (F) Representative PD-L1 immunohistochemical staining in lung adenocarcinoma and lung squamous carcinoma (200 ×). (G) Detection of PD-L1 expression in TOP2A-positive and TOP2A-negative groups by immunohistochemistry.
