Figure 1
From: Role of PDLIM1 in hepatic stellate cell activation and liver fibrosis progression
PDLIM1 is up-regulated in fibrotic liver tissues. (A) A schematic flow of the in vivo treatment. Mice (n = 12 mice per group) were challenged with CCl4 twice weekly at equal intervals for 6 weeks. (B) Masson trichrome staining in the livers of normal liver (n = 3) and fibrotic liver (n = 4) mice consecutively injected intraperitoneally with CCl4. (C) Immunohistochemical staining of PDLIM1 from normal liver (n = 3) and fibrotic liver (n = 4) mice. (D) Representative immunofluorescence images of α-SMA (red) and PDLIM1 (green) from normal liver and fibrotic liver mice. Nuclei are stained with DAPI (blue). Scale bars, 20 μm. (E) Western blotting analysis of PDLIM1 and α-SMA expression in liver tissues of three normal mice and four fibrotic mice. However, it should be emphasized that one of the protein extracts from fibrotic liver was degraded. Therefore, it is not represented in this figure. GAPDH was the loading control.
