Scientific Reports

Table 3 Dry matter intake (DMI), milk production expressed in energy corrected milk (ECM), and proportions of fatty acids (FA) as % of total FA from dairy cows supplemented with four different levels of iodoform (0, 320, 640, and 800 mg/day) intra-ruminally twice daily.

From: Enteric methane emission of dairy cows supplemented with iodoform in a dose–response study

	Iodoform supplementation				SEM	P-values
	0 mg/day	320 mg/day	640 mg/day	800 mg/day	SEM	Treatment	Linear effect	Quadratic effect
DMI in chambers, kg/day	22.5^a	20.3^ab	15.4^ab	11.7^b	2.46	0.04	< 0.01	0.32
Net energy intake, MJ/day	151.7^a	136.8^ab	103.8^ab	79.2^b	16.4	0.04	< 0.01	0.15
Milk, kg	31.7^a	30.8^ab	26.3^ab	20.2^b	3.26	0.04	0.01	0.12
ECM, kg/day	32.6^a	32.1^ab	27.7^ab	21.7^b	2.89	0.05	0.02	0.10
Energy balance¹, MJ/MJ	49.3	36.4	16.9	9.51	9.06	0.03	< 0.01	0.62
Fat, kg/day	1.28	1.29	1.14	0.91	0.105	0.06	0.03	0.08
Protein, kg/day	1.19	1.15	0.957	0.732	0.118	0.06	0.01	0.17
Lactose, kg/day	1.52^a	1.44^a	1.25^ab	0.961^b	0.137	0.02	< 0.01	0.12
Fat, %	4.11	4.29	4.40	4.49	0.336	0.26	0.03	0.91
Protein, %	3.81	3.75	3.64	3.59	0.285	0.12	0.02	0.50
Lactose, %	4.78	4.82	4.78	4.69	0.040	0.24	0.69	0.78
Urea, mM	0.492	0.493	0.480	0.429	0.0386	0.06	0.01	0.36
% of FA²
C14:0	12.9^a	12.3^ab	11.5^ab	10.0^b	0.592	0.03	< 0.01	0.32
C16:0	32.1	31.0	29.8	27.1	1.62	0.15	0.03	0.44
C18:0	8.84	9.67	9.57	10.85	0.864	0.21	0.07	0.81
C18:1 (cis-9)	21.3	21.5	24.2	28.4	1.85	0.06	0.03	0.16
SCFA	12.6	12.9	12.1	10.7	0.915	0.33	0.16	0.24
MCFA	50.9^a	48.8^ab	46.6^ab	41.5^b	1.93	0.03	< 0.01	0.29
LCFA	36.5^b	38.3^ab	41.3^ab	47.8^a	2.45	0.04	0.01	0.23
Saturated FA	74.0	73.0	70.2	65.3	2.34	0.08	0.02	0.21
Mono-unsaturated FA	25.2	25.0	27.5	31.6	1.73	0.07	0.04	0.12
Poly-unsaturated FA	3.63	3.87	3.68	4.00	0.246	0.64	0.46	0.97
Trans-unsaturated FA	2.83	3.23	2.85	3.65	0.461	0.47	0.39	0.85
De novo FA³	28.6	28.4	27.3	23.8	1.37	0.10	0.05	0.19
Mixed FA³	35.1	34.3	33.3	29.9	1.29	0.07	0.03	0.24
Preformed FA³	32.0^b	33.0^ab	35.6^ab	42.9^a	2.48	0.04	0.03	0.16

^abValues within the same line with different superscripts differ (P < 0.05).
¹Calculated as difference between net energy intake (MJ/d) and energy in milk (MJ/d).
²Fatty acids calculated as fat-% × 0.95, according to Schwarz et al.⁴⁵.
³Sum of de novo, mixed, and preformed FA do not add up to 100% as FA is calculated as fat-% × 0.95.

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