Figure 6

Direct contact assays of 3c{3,6} and 3c{6,6}. A. Number of dead mites (out of a total of five mites/replicate) treated with different doses of 3c{3,6}, counted at 2 h (blue), 4 h (red) and 6 h (grey) after start of the experiment. B. Number of dead mites (out of a total of five mites/replicate) treated with different doses of 3c{6,6}, counted at 2 h (blue), 4 h (red) and 6 h (grey) after start of the experiment. C. Total number of paralyzed and dead mites treated with different doses of 3c{3,6}, counted at 2 h (blue), 4 h (red) and 6 h (grey) after start of the experiment. D. Total number of paralyzed and dead mites when treated with different doses of 3c{3,6}, counted at 2 h (blue), 4 h (red) and 6 h (grey) after start of the experiment. A dose of 0 ng is the control treatment that received only ethanol. Bars represent mean ± S. E. of 5 replicates. Letters: comparison between each treatment dose and control at one time point- one-way ANOVA, followed by Tukey’s test, columns marked with different letters differ significantly p < 0.05: lower case xyz letters = 2 h, upper case ABC letters = 4 h and lower case abc letters = 6 h. Brackets above the letters indicate significant differences between the 2 h, 4 h and 6 h measurements for that dose—one-way ANOVA, followed by Tukey test (P < 0.05).