Table 1 Michaelis–Menten parameters of recombinant human acetylcholinesterase, hAChE, in the presence of compounds active against varroa.

From: Effects of dialkoxybenzenes against Varroa destructor and identification of 1-allyloxy-4-propoxybenzene as a promising acaricide candidate

Test compound

Vmax (× 10–5 M/min)

KM (× 10–4 M)

kcat (× 103 min−1)

None (control)

5.1 ± 0.2

1.0 ± 0.2

3.4 ± 0.2

0.5 mM DEET

6.5 ± 0.4

3.0 ± 0.4

4.4 ± 0.3

1 mM DEET

6.6 ± 0.7

4.0 ± 0.9

4.5 ± 0.5

3 mM DEET

7.9 ± 1.7

13.0 ± 4.0*

5.3 ± 1.1

0.5 mM 3c{3,6}

4.4 ± 0.3

2.0 ± 0.3

3.0 ± 0.2

1 mM 3c{3,6}

6.8 ± 0.6

4.0 ± 0.7

4.6 ± 0.4

3 mM 3c{3,6}

5.5 ± 1.0

3.0 ± 1.0

3.7 ± 0.7

0.5 mM 3c{6,6}

2.8 ± 0.2

1.0 ± 0.3

1.9 ± 0.1

1 mM 3c{6,6}

5.3 ± 0.4

2.0 ± 0.4

3.6 ± 0.3

3 mM 3c{6,6}

3.4 ± 0.5

0.7 ± 0.4

2.3 ± 0.3

0.5 mM 3b{2,2}

2.8 ± 0.7

0.6 ± 0.9

1.9 ± 0.5

1 mM 3b{2,2}

3.6 ± 0.3

0.8 ± 0.2

2.7 ± 0.2

3 mM 3b{2,2}

8.2 ± 1.2*

5.0 ± 2.0

5.6 ± 0.8*

  1. Values were obtained using GraphPad Prism 5 software analysis of rates obtained at different substrate concentrations using the Ellman assay (see text). Values shown are the kinetic constants obtained by least-squares fitting ± standard error. kcat was calculated by dividing Vmax by the concentration of AChE present in each 1 mL reaction (1.48 × 10–8 M).
  2. *Significant difference compared to all other treatment groups (Tukey, P < 0.05).
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