Table 2 Michaelis–Menten parameters of extracted honey bee Apis meliffera acetylcholinesterase, AmAChE, in the presence of compounds active against varroa.
Test compound | Vmax (× 10–6 M/min) | KM (× 10–5 M) | Specific activity (μM/min μg) |
|---|---|---|---|
None (control) | 9.8 ± 0.4 | 2.2 ± 0.5 | 0.44 ± 0.02 |
0.5 mM DEET | 10.9 ± 0.4 | 2.9 ± 0.5 | 0.49 ± 0.02 |
1 mM DEET | 11.0 ± 0.3 | 4.4 ± 0.5 | 0.49 ± 0.01 |
3 mM DEET | 9.7 ± 0.6 | 9.1 ± 1.8 | 0.44 ± 0.79 |
0.5 mM 3c{3,6} | 7.8 ± 0.3* | 2.0 ± 0.4 | 0.35 ± 0.01* |
1 mM 3c{3,6} | 6.7 ± 0.4*** | 2.9 ± 0.8 | 0.30 ± 0.02*** |
3 mM 3c{3,6} | 4.5 ± 0.2 *** | 0.8 ± 0.3 | 0.20 ± 0.01*** |
0.5 mM 3c{6,6} | 10.5 ± 0.3 | 1.7 ± 0.2 | 0.47 ± 0.01 |
1 mM 3c{6,6} | 10.6 ± 0.2 | 1.4 ± 0.2 | 0.47 ± 0.01 |
3 mM 3c{6,6} | 10.0 ± 0.3 | 1.9 ± 0.3 | 0.45 ± 0.01 |
0.5 mM 3b{2,2} | 10.6 ± 0.2 | 1.5 ± 0.2 | 0.48 ± 0.01 |
1 mM 3b{2,2} | 10.6 ± 0.2 | 1.3 ± 0.2 | 0.47 ± 0.01 |
3 mM 3b{2,2} | 12.9 ± 1.2*** | 2.7 ± 1.3 | 0.58 ± 0.05*** |
