Table 3 Michaelis–Menten parameters of extracted Varroa destructor acetylcholinesterase, VdAChE, in the presence of compounds tested against Varroa mites.
Test compound | Vmax (× 10−6 M/min) | KM (× 10−5 M) | Specific activity (μM/min μg) |
|---|---|---|---|
None (control) | 1.1 ± 0.1 | 1.4 ± 0.6 | 0.16 ± 0.01 |
0.5 mM DEET | 1.4 ± 0.1*** | 1.2 ± 0.4 | 0.20 ± 0.01*** |
1.0 mM DEET | 1.2 ± 0.1 | 1.9 ± 0.6 | 0.17 ± 0.01 |
3.0 mM DEET | 0.9 ± 0.1 | 5.4 ± 1.6*** | 0.13 ± 0.01 |
0.5 mM 3c{3,6} | 1.2 ± 0.1 | 1.2 ± 0.4 | 0.17 ± 0.01 |
1.0 mM 3c{3,6} | 1.3 ± 0.1 | 1.1 ± 0.3 | 0.19 ± 0.01 |
3.0 mM 3c{3,6} | 1.2 ± 0.1 | 1.2 ± 0.5 | 0.17 ± 0.01 |
3.0 mM 3c{6,6} | 0.8 ± 0.1** | 1.4 ± 0.5 | 0.12 ± 0.01** |
3.0 mM 3b{2,2} | 1.3 ± 0.1 | 1.4 ± 0.3 | 0.19 ± 0.01 |