Table 3 Michaelis–Menten parameters of extracted Varroa destructor acetylcholinesterase, VdAChE, in the presence of compounds tested against Varroa mites.

From: Effects of dialkoxybenzenes against Varroa destructor and identification of 1-allyloxy-4-propoxybenzene as a promising acaricide candidate

Test compound

Vmax (× 10−6 M/min)

KM (× 10−5 M)

Specific activity (μM/min μg)

None (control)

1.1 ± 0.1

1.4 ± 0.6

0.16 ± 0.01

0.5 mM DEET

1.4 ± 0.1***

1.2 ± 0.4

0.20 ± 0.01***

1.0 mM DEET

1.2 ± 0.1

1.9 ± 0.6

0.17 ± 0.01

3.0 mM DEET

0.9 ± 0.1

5.4 ± 1.6***

0.13 ± 0.01

0.5 mM 3c{3,6}

1.2 ± 0.1

1.2 ± 0.4

0.17 ± 0.01

1.0 mM 3c{3,6}

1.3 ± 0.1

1.1 ± 0.3

0.19 ± 0.01

3.0 mM 3c{3,6}

1.2 ± 0.1

1.2 ± 0.5

0.17 ± 0.01

3.0 mM 3c{6,6}

0.8 ± 0.1**

1.4 ± 0.5

0.12 ± 0.01**

3.0 mM 3b{2,2}

1.3 ± 0.1

1.4 ± 0.3

0.19 ± 0.01

  1. Values were obtained using GraphPad Prism5 software. Rates were obtained at different substrate concentrations using the Ellman assay. Specific activity was calculated by dividing Vmax by the weight of protein isolate present in each 1 ml reaction (6.86 μg).
  2. **Significant difference compared to all other treatment groups (P < 0.01), *** (P < 0.001).