Figure 3 | Scientific Reports

Figure 3

From: Fructooligosaccharide and Bacillus subtilis synbiotic combination promoted disease resistance, but not growth performance, is additive in fish

Figure 3

Scanning electron micrographs of intestinal microvilli and their density in L. fimbriatus fingerlings fed diets supplemented with fructooligosaccharide (FOS) and B. subtilis (BS) alone or in combinations for 60 days. (A) FOS (0.5% level), (B) LBS (104 CFU/g BS), (C) FOS + LBS (0.5% FOS and 104 CFU/g BS), (D) HBS (106 CFU/g BS), (E) FOS + HBS (0.5% FOS and 106 CFU/g BS) and (F) Control (without FOS or BS). (G) Microvilli density (average number of microvilli present on the surface of enterocytes standardised to a 4 μm2 region; means ± S.E.M: n = 4). (H) Microvilli diameter (average diameter of microvilli fed with FOS and BS supplemented diets alone or in combination; means ± S.E.M: n = 10). BS and FOS interactions effect are presented in the chart and bars sharing same small letter alphabet are not significantly different (p > 0.05). Main effects (FOS and BS) from Two-way ANOVA are presented in the results section. Significance of all tests was accepted at p = 0.05.

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