Figure 5 | Scientific Reports

Figure 5

From: Fructooligosaccharide and Bacillus subtilis synbiotic combination promoted disease resistance, but not growth performance, is additive in fish

Figure 5

Hematological changes in L. fimbriatus fingerlings challenged with pathogenic bacteria, A. hydrophila and fed diets supplemented with fructooligosaccharide (FOS) and B. subtilis (BS) alone or in combinations for 60 days. (A) White blood cells (WBC) count, (B) red blood cells (RBC) count, and (C) hemoglobin (Hb). Treatments: Con: Control: without BS/FOS, FOS: 0.5% FOS alone, Low BS(LBS): BS at 1 × 104 alone, FOS + LBS: 0.5% FOS and BS at 1 × 104 CFU, High BS (HBS): BS at 1 × 106 CFU alone, and FOS + HBS: 0.5% FOS and BS at 1 × 106 CFU. Data represented as means ± S.E.M. (n = 3). BS and FOS interactions effect are presented in the chart and bars sharing same small letter alphabet are not significantly different (p > 0.05). Main effects (FOS and BS) from Two-way ANOVA are presented in the results section. Significance of all tests was accepted at p = 0.05. Asterisks symbolize significant difference between (pre-) and (post-) challenge treatment groups (T-test; p < 0.05).

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