Figure 4

Restoration of cac1∆ rtt106∆-mediated chromatin assembly defects are dependent on transcription. Plasmid topoisomer distribution of the 2µ plasmid in wild type, rpb1-1, cac1∆ rtt106∆ and cac1∆ rtt106 rpb1-1 cells synchronized in G1 and released into fresh medium until mid-S phase (60 min for cac1∆ rtt106 rpb1-1 and 30 min for the rest) at 26 °C, and then shifted to and incubated with pre-heated fresh medium at 37 °C for the indicated times. Samples were run into different gels due to space limitations, and processed in parallel. Cell cycle progression and topoisomer profiles are shown. r and SC(−) indicate relaxed and negative supercoiling, respectively. Cropped images show only relaxed and negatively supercoiled topoisomers. Original gels are presented in Fig. S4B. The experiment was repeated twice with similar results.