Figure 4 | Scientific Reports

Figure 4

From: Effect of low-dose hydrocortisone and inhaled nitric oxide on inflammatory mediators and local pulmonary metalloproteinases activity in LPS-induced sepsis in piglets

Figure 4

MMP activities are reduced in the pulmonary tissue of iNO and IV hydrocortisone (iNO + HCT) exposed pigs. Gelatinolytic activity in pulmonary homogenates. (A) representative gelatin zymograms of pulmonary homogenates from the iNO + HCT group and the control group; densytometric analysis of MMP zymographic activity was performed using pulmonary homogenates from the pigs in the control group and from the iNO + HCT group. The optical density value for the pig with the lowest activity from the control group was set to 1, and then the results for all other animals were calculated accordingly. (B) An active form of MMP9 (60 kDa) and proMMP2 (72 kDa); (C) The total gelatinolytic activity quantified using a biotin–gelatin assay in pulmonary homogenates from the iNO + HCT and control groups. The measured mean absorbance A450 value for a pig with the lowest gelatinolytic activity was considered as 100%; results for all other animals were expressed as a percentage of this activity. Values are presented as means ± SEM. The Mann–Whitney U test was used for statistical analysis. *p < 0.05; **p < 0.005; ***p < 0.0005 vs. control group.

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