Figure 1 | Scientific Reports

Figure 1

From: Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells

Figure 1

Two wild-derived mouse strains (Mus musculus castaneus) were used in this study. CAST/Ei (A) and CASP/1Nga (B). Both strains have agouti coat color and smaller body weight than laboratory mice. (C) The phylogenetic tree of mouse subspecies strains. CAST and CASP divided from laboratory mice one million years ago, but within the same M. musculus species as domesticus (DBA/2 and B6). (D) Donor cell nuclei collected from caudal vein peripheral blood. Black arrowheads indicate leukocytes (putative granulocytes and monocytes) used as nuclear donors. White arrowhead: lymphocytes that have smaller cell sizes than granulocytes and monocytes. Bar = 20 µm. (E) ntESCs established from leukocytes SCNT embryos. Bar = 200 µm.

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