Figure 6

Chimeric mice and their offspring. (A) Appearance of chimeric mice (#12) derived from a CAST ntESC line (#M8) and (B6D2F1 × B6) F1 recipient embryos, which produced agouti and black coat colors, respectively. (B) Appearance of a chimeric mouse (#36) derived from a CAST ntESC line (#F2) and an (ICR × ICR) recipient embryos, which produced agouti and white (albino) coat colors, respectively. (C) Five F1 offspring between a B6 female and a CAST chimeric male (#23). Four pups with agouti coat color (#A3 to #A6, white arrowheads) carried the genome from the CAST ntESC line (#M8). The remaining black pup carried the host embryo genome ([B6D2F1 × B6]F1) (red arrowhead). (D) Microsatellite analysis of four F1 offspring (#A1, 2, 7, 8) generated by mating of a CAST chimeric male (#12 and #23) and B6 female, showing that the ntESC genome (#M8) was inherited to the F1 offspring. Microsatellite markers used are D1Mit126 (left) and D3Mit176 (right).