Table 1 Developmental efficiencies of SCNT embryos and ntESC derivation.

From: Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells

Strain (Species name)

Donor cell

Sex

No. embryos cultured

No. embryos at 24 h (%/No. cultured)

No. blastocysts (%/No. 2-cell)

No. established ntESC lines (%/No. blastocysts)

Fragmentation

2-cell

B6D2F1 (M. m. domesticus)

Cumulus

F

150

18 (12)a,b

127 (85)a

41 (32)a

6/17 (35)b

Leukocyte

M

96

30 (31)a′

50 (52)a′

13 (26)

8 (62)

Leukocyte

F

140

64 (46)a′,c

58 (41)a′,c,d

23 (40)c,d

14 (61)

CAST/Ei (M. m. castaneus)

Leukocyte

M

166

59 (36)b′

83 (50)a′

14 (17)a′

8 (57)

Leukocyte

F

88

33 (38)b′

51 (58)d′

4 (8)a′,c′

3 (75)

CASP/1Nga (M. m. castaneus)

Leukocyte

M

68

18 (26)

42 (62)

13 (31)

5 (38)

Leukocyte

F

88

24 (27)c′

56 (64)c′

10 (18)b′,d′

8 (80)b′

  1. a, a′ p < 0.01; b, b′ p < 0.05 as compared with B6D2F1 cumulus clone.
  2. c, c′ p < 0.01; d, d′ p < 0.05 as compared with same sex.