Table 1 Developmental efficiencies of SCNT embryos and ntESC derivation.
Strain (Species name) | Donor cell | Sex | No. embryos cultured | No. embryos at 24Â h (%/No. cultured) | No. blastocysts (%/No. 2-cell) | No. established ntESC lines (%/No. blastocysts) | |
|---|---|---|---|---|---|---|---|
Fragmentation | 2-cell | ||||||
B6D2F1 (M. m. domesticus) | Cumulus | F | 150 | 18 (12)a,b | 127 (85)a | 41 (32)a | 6/17 (35)b |
Leukocyte | M | 96 | 30 (31)a′ | 50 (52)a′ | 13 (26) | 8 (62) | |
Leukocyte | F | 140 | 64 (46)a′,c | 58 (41)a′,c,d | 23 (40)c,d | 14 (61) | |
CAST/Ei (M. m. castaneus) | Leukocyte | M | 166 | 59 (36)b′ | 83 (50)a′ | 14 (17)a′ | 8 (57) |
Leukocyte | F | 88 | 33 (38)b′ | 51 (58)d′ | 4 (8)a′,c′ | 3 (75) | |
CASP/1Nga (M. m. castaneus) | Leukocyte | M | 68 | 18 (26) | 42 (62) | 13 (31) | 5 (38) |
Leukocyte | F | 88 | 24 (27)c′ | 56 (64)c′ | 10 (18)b′,d′ | 8 (80)b′ | |