Figure 3
Supernatant of ascites upregulated signaling of active β-catenin. (A) Immunofluorescence for the localization of active β-catenin in MADOs treated by supernatant of ascites (A), exosome isolated from the same volume of the ascites (E) and the remaining part of the ascites (R). Scale bar, 100 μm. (B) Statistical scatter plots presenting average fluorescence intensity of MADOs treated above. Data indicated mean ± S.D. and analysed by Student’s t test (**p < 0.01; NS p > 0.05). (C) Histogram presenting relative luciferase activity of Topflash reporter in AGS-803 cells. Data indicated mean ± S.D. and analysed by Student’s t test (*p < 0.05; **p < 0.01; ***p < 0.001; NS p > 0.05).
