Figure 6

VPA does not alter offspring microbiota inter- and intra-diversity nor offspring microbiota relative abundance under both n-3 PUFA diets. Microbial diversity: (a) Chao1 index (first line) in dams (left), offspring males (center) and females (right). n = 5 mice per group. (b) Bray–Curtis index (third line) in dams (leftl), offspring males (center) and females (right). n = 10 (SAL/n-3 bal dam), 25 (VPA/n-3 bal dam), 25 (SAL/n-3 supp dam), 25 (VPA/n-3 supp dam), 10 (SAL/n-3 bal male), 25 (VPA/n-3 bal male), 20 (SAL/n-3 supp male), 25 (VPA/n-3 supp male), 10 (SAL/n-3 bal female), 25 (VPA/n-3 bal female), 25 (SAL/n-3 supp female) and 25 (VPA/n-3 supp female) mice. (c) Phyla abundance: Bacteroidetes abundance (first line) in dams (left), offspring males (center) and females (right). n = 5 mice per group except 4 in SAL/n-3 supp and VPA/n-3 supp male. Firmicutes abundance (second line) in dams (left), offspring males (center) and females (right). n = 5 mice per group except 4 in SAL/n-3 supp male and SAL/n-3 bal female. Data are expressed as median and min to max and were analyzed through a two-way ANOVA followed by Tukey post-hoc multiple analysis. *p < 0.05, **p < 0.01, ***p < 0.001.