Table 6 UV-absorbing compounds observed in extracts of only one of the two tree species based on the spectra presented in Fig. 1 and Supplementary Fig. S2. The compounds were putatively assigned to compound classes based on MS- and UV spectra and retention times.

From: Condensed tannins, novel compounds and sources of variation determine the antiparasitic activity of Nordic conifer bark against gastrointestinal nematodes

Extract source

Retention time[min]

Monoisotopic mass (Da)

Putative annotation (Compound or chemical group)

UV maxima (nm)

Spruce1

8.53

488.1457

Flavones and substituted flavones, eg. flavone glycosides

234, 289, 304, 323

8.54

423.1521

234, 289, 304, 323

9.41

456.1564

232, 287, 305, 321

11.03

504.1445

235, 288, 306, 322

11.15

514.1675

trihydroxy-flavan-dimer

238, 290, 302, 325

Pine

5.8

146.0367

Coumarin

237, 294, 311

7.0

218.0554 and 507.2309

Coumarin derivatives

231, 278, 305

16.2

226.1004

Pinosylvin monomethyl ether2

249, 299

  1. 1Annotated based on UV-spectra21,22,23 and occurrence only in the spruce bark extracts.
  2. 2Annotated based on24 and occurrence only in pine.
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