Table 4 Medial gastrocnemius gene expression.

From: Alterations in skeletal muscle morphology and mechanics in juvenile male Sprague Dawley rats exposed to a high-fat high-sucrose diet

Medial gastrocnemius

Gene (normalized to 18S)

CHOW Mean ± SD

HFS Mean ± SD

Effect size Cohen's d

p-value

Cellular stress

IL-6

0.03 ± 0.03

0.05 ± 0.02

0.78

p = 0.176

MCP-1

0.01 ± 0.01

0.02 ± 0.01

1.00

p = 0.316

TNFα

0.12 ± 0.08

0.18 ± 0.06

0.85

p = 0.053

Lipid droplet

Fsp27

0.76 ± 0.44

1.05 ± 0.66

0.52

p = 0.299

Leptin

0.26 ± 0.21

0.28 ± 0.10

0.12

p = 0.780

Adiponectin

1.06 ± 0.50

1.03 ± 0.35

0.07

p = 0.876

Cell death

IL-1β

0.03 ± 0.01

0.04 ± 0.01

1.00

p = 0.002

Cyto-C

1.58 ± 0.48

1.73 ± 0.22

0.40

p = 0.396

Caspase-3

0.62 ± 0.25

1.25 ± 0.33

2.15

p = 0.001

Muscle atrophy

MAF

1.27 ± 0.89

1.00 ± 0.25

0.41

p = 0.416

MURF

1.06 ± 0.70

0.81 ± 0.32

0.46

p = 0.369

Myogenesis

Pax7

0.68 ± 0.23

0.71 ± 0.38

0.10

p = 0.828

MyoD

0.42 ± 0.20

1.26 ± 0.55

2.03

p = 0.001

Myf5

0.30 ± 0.16

0.66 ± 0.44

1.09

p = 0.038

Myogenin

0.29 ± 0.11

0.71 ± 0.18

2.82

p = 0.001

MRF4

0.84 ± 0.45

1.16 ± 0.46

0.70

p = 0.162

  1. Data represent medial gastrocnemius gene expression normalized to 18S rRNA, assessed via qPCR. Bold indicates a statistically significant difference (p < 0.05).
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