Figure 2 | Scientific Reports

Figure 2

From: Anti-nanodisc antibodies specifically capture nanodiscs and facilitate molecular interaction kinetics studies for membrane protein

Figure 2

Avi-tagged MSP1D1 epitope mapping of seven monoclonal antibodies. To clarify the sequence recognized by the antibodies, the reactivity of the truncated and mutated peptides was tested. (A) The seven antibody epitopes identified with peptide arrays are shown on the respective avi-tagged MSP1D1 sequence. The spot intensity of the immunoreactivities is indicated by the line plots. (B) Alanine scanning analysis revealed amino acid residues that are suggested to be strongly involved in antibody-antigen binding because the spot intensity was significantly reduced owing to the mutations (shown in red). (C) The epitope of each antibody clone is shown in color: biND5 in red, biND8 in blue, and biND13 in green. Amino acid residues important for binding are shown in bold.

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