Table 1 Affinity against nanodisc (MSP1D1) and free MSP1D1.

From: Anti-nanodisc antibodies specifically capture nanodiscs and facilitate molecular interaction kinetics studies for membrane protein

Clones

Nanodisc (MSP1D1)

Free MSP1D1

KD (nM)

kon (1/Ms) × 104

koff (1/s) × 10−4

KD (μM)

kon (1/Ms) × 104

koff (1/s) × 10−4

biND5

5.03

4.27

2.05

0.832

0.572

25.2

biND7

16.7

4.83

8.04

4.10

1.66

681

biND8

71.1

5.56

39.6

3.39

0.882

299

biND10

16.2

2.59

4.20

17.5

0.207

362

biND12

63.9

0.706

4.51

729.0

0.00480

350

biND13

52.7

1.19

6.27

19.2

0.0797

153

biND14

96.4

2.41

23.2

635.0

0.00177

112

  1. The biND5 (bold) was the strongest affinity in this study, so we proceeded with further experiments.
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