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Figure 1

From: Identification of genes associated with the regulation of cold tolerance and the RNA movement in the grafted apple

Figure 1

RNA-seq experiment design and DEG analysis. (a) A scheme for cold tolerance signaling study. Stem vasculature tissues of ‘Gala’ apple scion grafted onto two contrasting rootstock cultivars showing a different level of cold tolerance (‘G202’, ‘M9’) were sampled at three winter stages: CA (early winter/cold acclimation, 6th June), DW (deep winter, 17th July), DA (late winter/cold de-acclimation, 26th August). (b,c) Principal component analysis of RNA-seq expression data. Top 5000 genes listed according to the variance rank were chosen as informative genes and used for PCA analysis: (b) rootstock (RS) and (c) scion (SC). (d) Venn diagram of DEGs between scion and rootstock with a criterion of |fold change|≥ 1.5 FDR ≤ 0.05. (e) RNA-seq validation by qRT-PCR. A high significant correlation (r ≥ 0.9) was made between RNA-seq result (RPKM) and qPCR.

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