Figure 2
From: Cytoplasmic HIF-2α as tissue biomarker to identify metastatic sympathetic paraganglioma
PPGLs express high levels of cytoplasmic HIF-2α and cytoplasmic HIF-2α is highly expressed in metastatic sPGLs. (A) Representative images from tumor cores scored as negative (0), weak (1 +), intermediate (2 +) or strong (3 +) for HIF-2α. (B) Quantification of positive cells for nuclear HIF-2α (HIF-2α-N) (in %). (C) H-score for cytoplasmic HIF-2α (HIF-2α-C). (D) Quantification of fraction (in %) of positive tumor cores for nuclear and cytoplasmic HIF-2α. (E) Quantification of fraction (in %) of tumor cores staining with high intensity (scored 2–3) for nuclear and cytoplasmic HIF-2α. (F)–(G) Expression of nuclear (HIF-2α-N) (F) and cytoplasmic (HIF-2α-C) (G) HIF-2α as divided by low (0–1) and high (2–3) staining intensity in sPGL, HN-PGL and PCC tumor subgroups. Tumor cores were scored manually by three independent researchers as well as semi-automatically using the QuPath-0.3.0 software. Statistical significance was determined by the Mann–Whitney test in B-C and Fisher’s exact test in D-G. sPGL, sympathetic paraganglioma; HN-PGL, head- and neck paraganglioma; PCC, pheochromocytoma. The scale bar represents 100 μm in core images and 50 μm in zoomed images. Note that there are no metastatic HN-PGLs in this cohort.
