Figure 6
Decreased apoptosis activation in OVE spheroids with p53R175H expression. (A) Representative immunoblots and (B) densitometric analysis of cleaved Caspase-3, cleaved Caspase-7, and cleaved Parp in OVE spheroids. Actin and vinculin were used as loading controls. (C,D) Caspase-3 and Caspase-7 activity in OVE spheroids determined by Caspase-Glo 3/7 assay. All statistical analyses were performed using one-way ANOVA followed by Tukey’s multiple comparisons test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n = 3). Error bars represent standard error of the mean. Original blots are presented in Supplemental Fig. S5.
