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Figure 3

From: Insights into peculiar fungal LPMO family members holding a short C-terminal sequence reminiscent of phosphate binding motifs

Figure 3

Kinetics and oxidative profile of the produced AA9-X282 on PASC. (A) Modularity of the studied AA9-X282s. Enzymes were named based on their organisms of origin: Coprinopsis cinerea (Cci), Irpex lacteus (Ila), Trametes ljubarskyi (Tlj), Pycnoporus coccineus (Pco), Trametes elegans (Tel), Schizophyllum commune (Sco). Each enzyme is associated with a color, consistently used in all panels. (B) HPAEC-PAD chromatogram of oxidized products released by the AA9-X282 (1 µM) from PASC (0.2%) in the presence of AscA (1 mM) after 24 h. In the control reaction the AA9 was replaced by CuSO4 (1 µM). (C) Time-course release of oxidized products (DP2ox + DP3ox) by CciAA9-X282-CBM1 and IlaAA9-X282-CBM1 (1 µM each) from PASC (0.2%) using ascorbic acid (1 mM) or PaCDHB (1 µM) as reductant. All reactions were incubated in sodium acetate buffer (50 mM, pH 5.2), in a thermomixer (850 rpm, 40 °C). Data points show average values and error bars show standard deviations (n = 3 independent biological replicates).

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