Figure 6
From: Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth
Enhanced neurite growth is supported by increased mitochondria and increased cellular respiration in A1EV-treated neurons. (A) Oxygen consumption rates (OCRs) of neurons treated with A1EVs, media alone, or EV-suppressed control media (GW4869: 20 µM and 40 µM). Focused analysis of basal respiration rates and spare respiratory capacity (B) and proton leak and ATP production are also shown (C). (D) Western blot analysis of the expression of the mitochondrial marker TOM70 in neurons following 12 h of A1EV or media only-treated neurons, to indicate mitochondria abundance. (E) Confocal images demonstrating TOM70 localization within A1EV or media only-treated neurons. Scale bar = 25 µm. * Indicates statistical significance from Control cells, p < 0.05.
