Table 2 Replicates of Ct and OD values for MB-AuNP-RT-I-PCR and Magneto-ELISA, respectively, for individual GUTB/non-TB control subjects.

Group		MB-AuNP-RT-I-PCR			Magneto-ELISA
Group		Ct1	Ct2	Mean Ct	OD1	OD2	Mean OD
Clinically suspected GUTB cases (n = 21)	1	9.42	9.3	9.36	0.362	0.363	0.3625
	2	10.3	10.25	10.275	0.326	0.321	0.3235
	3	10.6	11	10.8	0.312	0.309	0.3105
	4	11.2	11.4	11.3	0.168	0.165	0.1665
	5	12	11.9	11.95	0.302	0.301	0.3015
	6	13.8	13.6	13.7	0.172	0.174	0.173
	7	19.5	19	19.25	0.178	0.179	0.1785
	8	11.68	11.7	11.69	0.152	0.156	0.154
	9	12.45	12.48	12.465	0.298	0.294	0.296
	10	15.8	15.5	15.65	0.275	0.278	0.2765
	11	30.21	30.19	30.2	0.166	0.167	0.1665
	12	13.3	13	13.15	0.278	0.277	0.2775
	13	10.1	10.25	10.175	0.171	0.172	0.1715
	14	15.5	15.8	15.65	0.286	0.284	0.285
	15	30.18	30.16	30.17	0.176	0.179	0.1775
	16	12.8	12.84	12.82	0.281	0.283	0.282
	17	12.1	12.21	12.155	0.289	0.287	0.288
	18	11.49	11.62	11.555	0.172	0.172	0.172
	19	21.2	21.35	21.275	0.271	0.273	0.272
	20	15.78	15.85	15.815	0.329	0.327	0.328
	21	30.17	30.21	30.19	0.17	0.178	0.174
Non-TB controls (n = 20)	1	37.27	37.26	37.265	0.174	0.182	0.178
	2	27.48	27.49	27.485	0.179	0.176	0.1775
	3	31.71	31.75	31.73	0.178	0.165	0.1715
	4	29.07	29.17	29.12	0.187	0.172	0.1795
	5	28.69	28.59	28.64	0.182	0.179	0.1805
	6	32.56	32.68	32.62	0.181	0.178	0.1795
	7	21.18	21.3	21.24	0.209	0.198	0.2035
	8	34.26	34.22	34.24	0.167	0.178	0.1725
	9	29.01	29.13	29.07	0.178	0.175	0.1765
	10	31.06	30.96	31.01	0.195	0.162	0.1785
	11	34.09	34.21	34.15	0.181	0.177	0.179
	12	33.36	33.23	33.295	0.176	0.179	0.1775
	13	28.89	29.03	28.96	0.162	0.169	0.1655
	14	29.36	29.25	29.305	0.196	0.186	0.191
	15	36.69	36.82	36.755	0.166	0.179	0.1725
	16	38.25	38.14	38.195	0.158	0.176	0.167
	17	29.96	30.12	30.04	0.178	0.182	0.18
	18	30.03	29.88	29.955	0.134	0.167	0.1505
	19	34.25	34.37	34.31	0.125	0.146	0.1355
	20	33.6	33.46	33.53	0.135	0.116	0.1255

From these data, the mean Ct values lesser than NC+3SD (26.20) were considered to be positive for GUTB cases by MB-AuNP-RT-I-PCR, whereas the mean Ct values greater than 26.20 were considered as negative for non-TB controls. Meanwhile, the mean OD values greater than ‘mean OD of non-TB control group+2SD’ (0.189) were considered to be positive for GUTB cases by Magneto-ELISA, while the mean OD values lesser than 0.189 were considered as negative for non-TB controls.

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