Table 4 Histomorphology of the different intestine sections of European seabass fed the control or the blend diets using a semi-quantitative analysis.

From: An in-depth characterisation of European seabass intestinal segments for assessing the impact of an algae-based functional diet on intestinal health

 

Anterior intestine

Mid intestine

Posterior intestine

Rectum

CTRL

Blend

CTRL

Blend

CTRL

Blend

CTRL

Blend

Villi length and integrity

1.9 ± 1.3

3.2 ± 2.0

2.4 ± 1.4

4.4 ± 2.5

2.1 ± 1.3

2.6 ± 1.2

1.4 ± 0.7

2.2 ± 1.2

Submucosa width

1.4 ± 0.5

2.0 ± 1.3

2.4 ± 1.0 b

4.3 ± 2.1 a

2.2 ± 0.7

3.1 ± 2.2

1.8 ± 0.8

2.4 ± 0.5

Lamina propria width

1.9 ± 0.9

3.2 ± 2.1

1.9 ± 0.9 b

3.1 ± 1.3 a

1.4 ± 0.7

1.4 ± 0.7

1.2 ± 0.4

1.2 ± 0.4

Submucosa lymphoid cells

2.3 ± 1.1

2.3 ± 1.1

2.6 ± 1.1 b

3.4 ± 0.7 a

2.2 ± 0.9

2.9 ± 0.9

2.4 ± 1.0 b

3.4 ± 0.7 a

Submucosa granulocytes

2.5 ± 1.2 b

3.5 ± 1.0 a

2.2 ± 0.9 b

3.6 ± 1.0 a

1.9 ± 0.9

2.7 ± 0.7

2.1 ± 0.7 b

2.8 ± 0.4 a

Lamina propria lymphoid cells

1.9 ± 0.6

2.2 ± 1.1

2.5 ± 0.7 b

3.4 ± 0.7 a

2.8 ± 1.0

3.1 ± 1.0

2.4 ± 0.8

2.5 ± 1.1

Lamina propria granulocytes

2.4 ± 1.0

3.4 ± 1.1

2.2 ± 0.8

2.3 ± 1.3

2.1 ± 0.7

2.4 ± 0.5

1.5 ± 0.5

1.4 ± 0.5

  1. CTRL – fish fed the control diet, Blend – fish fed the blend diet. Scores 1–9 were assigned to villi length/integrity, submucosa width, and lamina propria width; while scores 1–5 were assigned to denote the presence of leucocytes (i.e., lymphoid cells and granulocytes) in the submucosa and lamina propria. Values presented as mean ± SD (n = 10 samples per dietary treatment), based on a semi-quantitative analysis. Independent samples t-test was employed to detect significant differences between the groups within each intestinal section. Different letters denote significant differences between dietary treatments.
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