Table 1 Primer sequences and probes used in this study.
Genes | Forward (5′–3′) | Reverse (5′–3′) | |
|---|---|---|---|
SYBR green PCR system | |||
IFN-γ | GAAAGCCTAGAAAGTCTGAAGAAC | GCACCGACTCCTTTTCCGCTTCCT | |
IL-6 | TGATGGATGCTTCCAAACTG | GAGCATTGGAAGTTGGGGTA | |
IL-1β | CACCTTCTTTTCCTTCATCTTTG | GTCGTTGCTTGTCTCTCCTTGTA | |
CCR2 | TTCTGGGCTCACTATGCTGC | AAGGGCCACAAGTATGCTGA | |
Bax | CCAGGACGCATCCACCAAGAAGC | TGCCACACGGAAGAAGACCTCTCG | |
Caspase-1 | GTGTTGCAGATAATGAGGGC | AAGGTCCTGAGGGCAAAGAG | |
Caspase-3 | GGACCTGTGGACCTGAAAAA | GCATGCCATATCATCGTCAG | |
Bcl-2 | GGATGACTTCTCTCGTCGCTACCGT | ATCCCTGAAGAGTTCCTCCACCAC | |
NFκB | GCATGCCATATCATCGTCAG | TGCTTCTCTCCCCAGGAATA | |
OB-R | TGCCTTGGAGGACTATGGGT | AGCCCCCTTCAAAGACGAAG | |
ACC | GCCTCTTCCTGACAAACGAG | TCCATACGCCTGAAACATGA | |
SREBP-1c | TGGATTGCACATTTGAAGACAT | GCTCCTCTTTGATTCCAGGC | |
FAS | CAGCTGTCAGTGTAAAGAAACATGTC | AGCTCACGTGCAGTTTAATTGTG | |
HMGCR | CCCAGCCTACAAACTGGAAA | CCATTGGCACCTGGTACTCT | |
SREBP-2 | AGACTTGGTCATGGGGACAG | GGGGAGACATCAGAAGGACA | |
18S | ATGAGTCCACTTTAAATCCTTTAACGA | CTTTAATATACGCTATTGGAGCTGGAA | |
Genes | Forward (5′–3′) | Reverse (5′–3′) | Probe |
|---|---|---|---|
Taqman probe PCR | |||
TNF-α | AATGGGCTCCCTCTCATCAGT | ACGGGCTTGTCACTCGAGTT | CCAGACCCTCACACTCAGATCATCTTCTCA |
iNOS | GGACATTAACAACAACGTGGAGAA | AACCATTTTGATGCTTGTGACTCTT | TGCTATTCCCAGCCCAACAACACAGG |
OPN | CAAAGTCCAGGAGTTTCCCTGTT | CTCTTCATGCGGGAGGTGA | TGATGAACAGTATCCCGATGCCACAGAT |
18S | ATCCATTGGAGGGCAAGTCTGGTGC | ATGAGTCCACTTTAAATCCTTTAACGA | CTTTAATATACGCTATTGGAGGCTGGAA |
