Figure 5
Effect of pH on enzyme activity (-●-) was done by adjusting the reacting mixtures to different pHs (a). While the pH stability (-○-) was examined by preincubation of enzyme at different pHs, then residual activity against colloidal chitin was determined (a). The effect of temperature on chitinase activity (b) was studied by incubation of the reacting mixtures at different temperatures. While the thermal stability (c) was determined by exposing chitinase to 50–80 °C for 15–60 min then assayed for residual activity against colloidal chitin. The midpoint temperature (Tm) at which the tested chitinase lost half of its activity was calculated from (d).
