Figure 2 | Scientific Reports

Figure 2

From: Expandable ELAST for super-resolution imaging of thick tissue slices using a hydrogel containing charged monomers

Figure 2

Microscopic distortion analysis of ExELAST strategy. (a–f) Tubulin of BS-C-1 cells was stained and imaged. (a,b) Before and after expansion image of proExM. (c,d) Before and after expansion image of the first hydrogel composition. (e,f) Before and after expansion image of the second hydrogel composition. (g) Pre-synaptic marker (Bassoon, red), and post-synaptic marker (Homer1, green) of mouse brain slices were stained and imaged with the first hydrogel composition. (h) Homer1-Bassoon distance profile of the expanded image. All the images were obtained with a 40× water immersion objective lens, 1.15 NA.

Back to article page