Figure 3
A comparison of immunostaining depth in non-stretched state and stretched state of ExELAST hydrogel. Myelin Basic Protein (MBP) of mouse brain slices was stained and imaged. For (a–d), staining was performed without stretching. (a) Side view of MBP single channel z-stack image using 3D projection function of Fiji software. (b) Myelin sheath image acquired at the surface. (c) Myelin sheath image acquired at the center of the depth. (d) Normalized intensity of top 0.5% brightest pixels of each slice for MBP channel. For (e–h), staining was performed without stretching. (e) Side view of MBP single channel z-stack image using 3D projection function of Fiji software. (f) Myelin sheath image acquired at the surface. (g) Myelin sheath image acquired at the center of the depth. (h) Normalized intensity of top 0.5% brightest pixels of each slice for MBP channel. All the images were obtained with a 10× dry objective lens, 0.45 NA.
