Figure 4 | Scientific Reports

Figure 4

From: Expandable ELAST for super-resolution imaging of thick tissue slices using a hydrogel containing charged monomers

Figure 4

Immunostaining of thick mouse brain slices through ExELAST. (a,b) Maximum intensity projection (MIP) image of fully stained 500-μm-thick mouse brain slice. (c) Image acquired at the surface. (d) Enlarged image of yellow boxed region in c. (e) Image acquired at the center of the depth. (f) Enlarged image of yellow boxed region in e. Blue: 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), red: Homer1, green: Bassoon. All the images were obtained with a 60× water immersion objective lens, 1.00 NA.

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