Figure 3
From: Defects in early synaptic formation and neuronal function in Prader-Willi syndrome
Neuronal differentiation induced from control, M-iPWS, NDN KO, and MAGEL2 KO iPS cells. Quantitative mRNA analysis of neuronal markers (ßIII-tubulin and MAP2) (A) and synapse-related genes (presynapse, SYN1; postsynapse, PSD-95 and SLITRK1) (B). Each value was normalized to the GAPDH value and expressed as the fold induction from the levels in undifferentiated control iPS cells. Values represent the mean ± S.D. All data were evaluated by one-way ANOVA followed by Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (all data, n = 7). (C) 14-day differentiated neurons were immunostained with neuron (ßIII-tubulin) and DNA (DAPI). The scale bars represent 10 µm.
