Figure 4
From: Defects in early synaptic formation and neuronal function in Prader-Willi syndrome
Synaptic density on neurons derived from control, M-iPWS, NDN KO, and MAGEL2 KO iPS cells. (A) Representative 2D reconstruction of PSD-95 puncta on βIII tubulin-positive dendrites for each neuron. Neurons are indicated in green, and colocalization of PSD-95 is indicated in red. All scale bars represent 5 µm. (B) Quantification of PSD-95 puncta on neurons. PSD-95 per 10 µm neuron: control, 14 ± 3.5; M-iPWS, 9.9 ± 2.9; MAGEL2 KO; 9.3 ± 3.0; NDN KO; 7.2 ± 2.0. Values represent the mean ± S.D. All data were evaluated by one-way ANOVA followed by Tukey’s multiple comparisons test, ****p < 0.0001 (all data, n = 30; independent neurons of several experiments).
