Figure 5

(A–C) DBSCAN estimated molecular clusters for data obtained at short exposure time along with standard SMLM and large exposure time (\(50~{\text {ms}}\)). (D) An overlay of the clustered localizations, color-coded by the integration time. (E)The plots show average value of estimated biophysical parameters (number of HA molecules per μm2, number of molecules per cluster (\(\# N / C\)), cluster area (μm2) and clustered fraction \(\sum _i C_i / C_A\) ) relevant to formed molecular clusters, where \(C_i\) and \(C_A\) correspond to total cluster area and cell area, respectively.