Figure 1 | Scientific Reports

Figure 1

From: Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct

Figure 1

BRD4-NUT fusion protein forms nuclear condensates in human cells. (a) Western blot showing the expression levels between stable cell lines expressing different constructs. All cell lines treated with 5 ng/mL Doxycycline for 2 h, followed by 4 h washout. Antibodies used include BRD4(N) and GAPDH as a loading control. (b) Cartoon of BRD4, NUT and BRD4-NUT fusion proteins. Bromodomains 1 and 2 and extraterminal domain of BRD4 as well as predicted α-helices are indicated. (c) Line profiles across cells expressing mNeonGreen-tagged BRD4, NUT and BRD4-NUT fusion; scale bars = 10 μm. (d) Micrographs of cells expressing mNeonGreen-tagged BRD4, NUT and BRD4-NUT; scale bars = 10 μm. (e) Quantification of percentage of cells forming condensates (at least 2 condensates larger than 1.25 μm in diameter).

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