Figure 5

Relative expression levels of the esterase gene CHU_2040 in Pleurotus pulmonarius using the most (EF1α and PHOS; EF1α and MNP3) and the least (LAC and VP) stable pairs of reference genes. RT-qPCR was performed with three biological replicates, considering the combined condition C (datasets for P. pulmonarius cultivated on toxic and non-toxic jatropha cake). (A) EF1α and PHOS; (B) EF1α and MNP3; (C) LAC and VP.